Control of Gene Expression in eukaryotes, Factors involved in the regulation of gene transcription. factors involved in Chromatin remodelling. Gene silencing and micro RNA. Organism models useful in molecular biology. Cell Division Cycle and regulatory factors of cell proliferation and cell arrest. Apoptosis. Differentiated Cells and the Maintenance of Tissue. Cytoskeleton structure and remodelling. Cell Junctions, Cell Adhesion and Cell motility and the Extracellular Matrix
Alberts et al. Biologia Molecolare della cellula Ed. Zanichelli
Darnell, Lodish: Biologia molecolare della cellula Ed. Zanichelli
T.A. Brown Genomi Ed. Edises
Lewin Benjamin; Il gene VIII; Ed. Zanichelli
R.F. Gesteland and J.F. Atkins, The RNA World -
J. Watson, Biologia Molecolare del Gene . Ed. Zanichelli
Learning Objectives
Knolewdge acquired:
Regulation of gene expression in eukaryotes and techniques for gene expression evaluation. Molecular aspects of the principal biological processes: cell growth, differentiation, cell death and motility and specific techniques for their evaluation
Competence acquired
Terminology. Basic aspects and molecular factors involved in the control of the fundamental biological processes: cell growth, differentiation, death, motility and adhesion.
Molecular factors of the signalling pathways leading to cell survive and death.
Skills acquired (at the end of the course):
To identify the appropriate tecniques for evaluating gene expression, cell proliferation, death, differentiation, motility in the eukaryotes
To develop skills for critical analyses and synthesis of acquired information.To acquire abilities for communication of scientific information.
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Prerequisites
Courses to be used as requirements (required and/or recommended)
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Courses required:
Courses recommended: Biochemistry, genetics, Cell biology
Teaching Methods
Intermediate examinations: 0Stages: 0Seminars (hours): 0Contact hours for: Laboratory-field/practice (hours): 0Contact hours for: Laboratory (hours): Contact hours for: Lectures (hours): 48 Hours reserved to private study and other indivual formative activities: Total hours of the course (including the time spent in attending lectures, seminars, private study, examinations, etc...): 150 (=6x25)
Further information
Frequency of lectures, practice and lab:
The attendance of the lessons is strongly recommended.
Teaching tools
The instruments and facilities at the Department of Biochemical Sciences as well as a Laboratory of Cell Biology specific for practical lessons are used.
Monday 5-6 pm
Dept. of Biomedical Sciences – Biochemistry and Molecular Biology Section , Viale Morgagni 50- 50134 Firenze.
Students will be received in other days after e-mail or phone contact.
Type of Assessment
Oral exam
Course program
Regulation of gene expression in eukaryotes. Structure and regulation of transcription factors: Chromatin remodelling (Swi sfn complex, HMG proteins). Epigenetic factors: post-translation modifications of histones, protein folding. Gene silencing and microRNA: processing and biological means. Transient, semipermanent and permanent regulation of gene expression. Cell specification and differentiation. Molecular aspects of the principal biological processes: cell growth, differentiation, cell death and motility. Adesion molecules. Extracellular matrix remodelling. Cytoskeleton: structure and function. Cell communication: gap junctions. Methodologies for the evaluation of cell growth, cell death and cell migration.