Principles of genetic analysis: Mendel, extensions of Mendelian analysis. Quantitative genetics. Population genetics: allelic frequencies and evolution of populations. Regulation of gene expression in Prokaryotes and Eukaryotes. Organism differentiation. Epigenetics. Genomes: sequencing, anatomy. Evolution of genes and genomes; molecular phylogeny.
Russel P.J., Genetica (terza edizione), Pearson.
Brown T.A. Genomi 3, EdiSES. Meneely P. Analisi genetica avanzata, McGraw-Hill. Deho G. e Galli E., Biologia dei microrganismi, Casa editrice ambrosiana. Lewin B. Il gene VIII, Zanichelli.
Learning Objectives
Knowledge acquired:
Knowledge of advanced genetics of cell, organism and population. Understanding the role of gene regulation on organisms differentiation, including bacteria.
Basic knowledge of molecular mechanisms of evolution.
Competence acquired
Principles of genetic analysis: Mendel, extensions of Mendelian analysis. Quantitative genetics. Population genetics: allelic frequencies and evolution of populations. Regulation of gene expression in Prokaryotes and Eukaryotes. Organism differentiation. Epigenetics. Genomes: sequencing, anatomy. Evolution of genes and genomes; molecular phylogeny.
Skills acquired (at the end of the course):
Application of genetic methods to the understanding of biological and biochemical issues. Use of mutants and genetic crosses in model organisms. Usage of genetic models for studying development and differentiation. Comprehension of the evolutionary models and techniques of genomic analysis.
Prerequisites
Basic genetics, molecular biology and microbiology
Teaching Methods
Total hours of the course (including the time spent in attending lectures, seminars, private study, examinations, etc...): 150
Hours reserved to private study and other indivual formative activities:
Contact hours for: Lectures (hours): 48
Contact hours for: Laboratory (hours): None
Contact hours for: Laboratory-field/practice (hours): None
Seminars (hours): 0
Stages: 0
Intermediate examinations: 0
Further information
Frequency of lectures, practice and lab:
Highly recommended.
Teaching tools
Additional teaching material will be provided during lectures.
Office hours:
Friday 10:00-12:00
By appointment
0554574743
marco.bazzicalupo@unifi.it
Type of Assessment
Written and oral examination
Course program
Course Contents (detailed programme):
Principles of genetic analysis: Mendel, Morgan, genetic crosses and genetic mapping; extension of Mendelian analysis; human genetics.
Quantitative genetics: continuous traits, how to measure them; statistical tools, the search for responsible genes, QTL.
Population genetics: principles, Hardy-Weinberg law, analytical tools, applications; determination of allelic frequency; evolution of populations.
Regulation of gene expression:
Prokaryotes; cell cycle, differentiation, cell communication
Eukaryotes: control mechanisms, development of fly and worm.
Epigenetics.
Genomes: sequencing, structure, comparative genomics.
Evolution: the origin of life on earth, evolution of genes and genomes in prokaryotes and eukaryotes; molecular phylogeny; the extended synthesis.