The course focuses on main experimental techniques that are available to scientific research , both in molecular and cellular fields, that involve recombinant DNA and antibody technologies. It also shows implications for gene therapy and forensic sciences.
Molecular Biotechnology: Principles and Applications of Recombinant DNA
Glick and Pasternak ASM Press
Molecular Biology of the Cell,
B. Alberts, et al., Garland Science
Learning Objectives
The student has a deep overview of techniques that are available for scientific research
Prerequisites
Basic knowledge of molecular biology and biochemistry
Teaching Methods
Frontal lessons. Lab experience
Type of Assessment
Oral examination
Course program
Eukariotic expression vectors.
Methods for genic expression determination. Determination of heritary pathology-linked genes and gene therapy strategies. Method for studying the cell cycle: cell cycle checkpoints and cell synchronization. Site-directed mutagenesis. Co-localization of cellular proteins: co-immonoprecipitation and FRET .
Signals from extracellular matrix. Epithelial-mesenchymal transition. Anoikis.
ChIP in DNA-protein interaction studies. RNA-protein Interaction as a regulative strategy for mRNA stabilization and function. In vitro techniques for cell transformation and apoptosis studies.
2CFU are devoted to the pratical approach to main cellular and molecular biology techniques