Text book:
Biochimica e biologia molecolare. Principi e tecniche. di K. Wilson and J. Walker. Raffaello cortina editore
Reviews from PubMed, dealing with main topics
Learning Objectives
Knolewdge acquired:
Preparation of samples, extraction, purification. Spectrophotometry: absorbance and fluorescence. Chromatography and electrophoresis: principles and advanced techniques. The set up and management of a biochemical laboratory. Proteomics techniques: 2D electrophoresis and 2D-chromatography, mass spectrometry. Differential and functional proteomics. Clinical and environmental application of Proteomics.
Competence acquired
Lessons have the aim to show the student the right methodology for the analytical and preparative determination of proteins and enzymes, . The analysis of the obtained results is also discussed. Theoretical and practical aspects of a proteomic study.
Skills acquired (at the end of the course):
Students will be able to set up experiments of protein purification, electrophoresis of DNA and proteins, western blot. 2D electrophoresis techniques and sample preparation.
Prerequisites
Courses to be used as requirements (required and/or recommended)
Slides of lessons will be downloaded on Moodle.unifi. The required password will be provided to the students during lessons.
Further information
Frequency of lectures, practice and lab:
the frequency is strongly suggested.
Teaching tools
The instrumentation of the Department of Biochemical Sciences is using to perform demonstrative lessons and practice.
Office hours:
Wednesday: 11-13 a.m. and 16-18 p.m. at Dept. of Biochemical Sciences, Viale Morgagni 50
Students can be received in other days after e-mail appointment.
Type of Assessment
oral examination about the course contents and critical lecture of a scientific paper.