PREVIO CONTATTO IBBR-CNR, Polo Scientifico, Via Madonna del Piano 10 Sesto Fiorentino (FI)-50019
TEL 0555225786
https://ibbr.cnr.it/ibbr/info/people/mariella-barattihttps://www.unifi.it/p-doc2-2010-0-A-2c2a34313428-0.html Web of Science: Researcher ID AAX-1970-2020https://orcid.org/0000-0002-6336-8924matricola Docente UNIFI: D100821
ACTUAL POSITION Permanent Researcher, Research National Council-CNR, Matricola 9757 Professor on contract LM60 Science and Technology of Nature and Man, University of Florence, Course “Management of projects for the protection of fauna" (B029168).
WORK EXPERIENCE
From 2009 to present
From 2009 to 2005
2007
From 2005 to 2004
From 2004 to 2001
From 2000 to 1998
From 1998 to 1996
Permanent researcher PROVV 7/73 prot n. 1809 del 26.11.2007
Art. 23 CNR Temporary Researcher Prot. N 7035 del 29/12/2005
Competition winner Tuscany Region upper secondary school class A059, A060, Professor on payroll 2007
Art. 15 CNR Temporary Researcher Prot. N. 004/2014 del 31/3/2004
Research Contractor: Department of Animal Biology and Genetics "Leo Pardi", University of Florence. Project: Use of molecular techniques for the characterization of genetic variability in birds. Study of Major Histocompatibility Complex (MHC) in relation with sexual selection, MHC genotypes and mate choice in GalliformesScientific Sector: BIO/05Supervisor: Prof. Francesco Dessì-Fulgheri Research Activity: Inter and intrapopulation genetic variability in birds, with particular attention at Galliformes, comparison between bred and wild populations, monitoring of reintroduction and artificial hybridization. Acquired techniques: DNA preservation and extractions of different animal tissues, microsatellite genotyping, Sanger Sequencing, Cloning, CE-SSCP analysis, Statistical methods applied to phylogeography, Molecular Phylogeny, MHC genotypes
Post-Doctoral Fellowship prot. N. 2762 of 20/9/1996University of Florence, Department of Animal Biology and GeneticsProject: Genetic variability in birds. Foundation of the first molecular lab at Department of Animal Biology and GeneticsScientific sector: BIO/05Supervisor Prof. Francesco Dessì-FulgheriResearch Activity: Inter and intrapopulation genetic variability in birds, with particular attention at Galliformes, comparison between bred and wild populations, monitoring of reintroduction and artificial hybridization. Acquired techniques: DNA preservation and extractions of different animal tissues, RAPDs, Sanger Sequencing Tuscany Regional Fellowship Prot. 9283 del 18/10/1993University of Siena, Department of Evolutionary BiologyProject: Study of soil fauna and its role in the degradation of organic matterScientific sector: BIO/05Supervisor: Prof. Romano Dallai Research Activity: study of the relationship between environmental pollution action and damage to biocoenosis in Tuscany forest ecosystems. acquired techniques: morphological identification (optical and electronic microscopy) of taxa present and statistical analysis of qualitative and quantitative data. use of software for the statistical study applied to ecological data.
EDUCATION AND TRAINING
1989 Post-degree Specialization in Agriculture AcarologyOhio State University
From 1992 to 1988 PhD in Animal Biology (Zoology), IV cycleUniversity of Siena Project: The Oribatid mites of Tuscany ArchipelagoScientific sector: BIO/05Supervisor: Prof. Fabio Bernini Research Activity: Systematic, ecological and biogeographical analyses of Oribatid mites. Acquired techniques: optical microscopy, scanning electron microscopy (Philips 550 and XL20), basic techniques for transmission electron microscopy; allozyme electrophoresis (agarose and acetate), DNA analyses
From 1982 to 1988Degree in Natural Sciences (110/110 cum laude)University of FlorenceTitle: “The aquatic subterranean fauna of Apuan Alps” Scientific sector: BIO/05Supervisor: Dr. G. Messana Research Activity: Spring, well and cave samplings, methods of collecting groundwater and phreatic fauna, characterization of species and their abundances, Biogeography and morphological investigations
Committee
Abroad Experiences 2015-2012 Consiglio Scientifico del Parco Regionale di San Rossore Massaciuccoli2012-2010 CITES (Convention on International Trade of Endangered Species) Ministero dell’Ambiente e del territorio, Direzione Protezione della Natura1998: Department of Animal Sciences, Animal Breeding and genetics Group, Study University of Wageningen (NL), lab activity for isolation of microsatellites for Galliformes (Dr. MAM Groenen)1989 Department of Environmental and Forest Biology Syracuse University (N.Y. State, U.S.A.); cellulose acetate electrophoresis for the study of allozymes (Prof. Norton).
Peer reviewing of International Journal
Ongoing and last projects
Pre and post academic teaching Neuroscience & Behavioral Reviews, Proceedings of The Royal Society Of London, Part B Tropical Zoology, Ecology Ethology and Evolution, Behavior Ecology and Sociobiology, Hydrobiologia, Plos One, Biological Invasions, Biological Journal of Linnean Society, Tropical Zoology, Journal of Biological Research, Behavioral Ecology and Sociobiology, Frontiers in Zoology, Canadian Journal of Zoology, Journal of Biogeography
-CNR Cooperation projects CNR / CNRST Italy-Morocco 2020-2021 The stone-curlew Burhinus oedicnemus (Aves, Charadriifromes) as a model for the study of the possible impacts on biodiversity of the process of intensification of agricultural practices in Morocco Principal investigator. Partners University of Agadir; Università di Pisa (Dipartimento di Biologia, Dipartimento di Scienze Veterinarie) -CNR Cooperation projects CNR / CNRST. Italy-Morocco 2018-2019 Multidisciplinary study of the species Burhinus oecdinemus (Aves: Charadriiformes: Burhinidae), in Morocco for estimating the effects of climate change in the Mediterranean biodiversity hotspot”. Principal investigator partners University of Agadir; Università di Pisa (Dipartimento di Biologia). -“Balneazione Lamma” Support in activities related to the management of marine protected areas (AMP) close to port areas, with the ultimate aim of developing management plans that allow the effective protection of AMP. 7676 del 15/10/2018. Principal investigator-Tuscan Archipelago/ LIFE13 NAT/IT/000471 2015-2016: "Genetic survey on the populations of european hedgehog of the Tuscan archipelago– RESTO CON LIFE" – CUP: E96J13001020007. Principal investigator. Partners: ISPRA-FishTrack AGRIFOOD Tuscany Region 2014-2016, PRAF 2012-2015 Measure 1.2. Analytical techniques for the unmasking of food fraud to guarantee and protect the regional fish heritage, coordinator of operational unit principal unit: Department of Evolutionary Biology University of Siena-Autonomous Region of Sardinia 2013-2015, Department of Programming, Budget, Credit and Land Planning: "Genetic investigation on the lepidopter-oak association in Sardinia". Partner, Department of Evolutionary Biology of Siena, ISE, CNR PGI-POR FESR 2012-2010 Activities 1.1 action line a regional call 2008 for support for joint research projects between groups of companies and research bodies in the field of environment, transport, logistics, infomobility and energy, "reduction of the impact of agricultural pressures on Water Resources" (R.I.P.A.R.I.), Partner, principal coordinator: Hydrogea, partners ETG srl-RSTL/CNR Free-themed Spontaneous Research n. 59, 2010-2007 The underground island ecosystem as a model for the study of biodiversity. Principal investigator-PRIN 2008 2X5YZC: "interactions between MHC genotype and androgen-mediated maternal effects in determining phenotype and sexual choice in the common pheasant phasianus colchicus".partner, principal coordinator: Università statale Milano, Prof. Saino-PRIN 2005 219/2005, Genetic variability to MHC loci: Comparison between populations in different classes of vertebrates to identify the role of selective and demographic processes", partner,leader of The University of Padua, Prof. Patarnello, partners University of Florence, Padua and Ferrara-FIRB 2001 RBAU01H5C3: Study and comparison of the genetic structure of marine species of tropical mangrove crustaceans, characterized by different reproductive strategies. partners University of Florence- Forest Park Casentino Authority, 2003 229/4/2003 Genetic characterization of trout of the Casentino Forests National Park, participant, university partner of Florence- Tuscany Region 2005-2002, n. 5795/2002 "Eco-ethological and genetic characterization of Tuscan, wild and domestic populations, of Mallard", participant, partners: University of Florence-PRIN 31/2000 2003-2001 The behaviour as a biomarker of the effects of estrogenic pollutants in higher vertebrates. Partners. Partners University of Florence, UNITO, UNISI, UNIPR-RSTL/CNR Free-Themed Spontaneous Research N 23, 2008-2005 The colonization processes of terrestrial and freshwater environments In Crustaceans, Partner
Pre-degree TeachingLectures and Lab practice for the degree courses in Biological and Natural Sciences at University of Siena, a.a. 1988-1996.Lectures and lab practice for the degree courses in Applied Zoology, Biological and Natural Sciences at University of Florence, a.a. 1997-2006.Lectures for the specialized degree in "Animal Sciences and Animal Technologies" LM86, Faculty of Agriculture, University of Florence, 2004-2013.Molecular Zoology Laboratory" Course LM60 Natural Sciences, Science of Nature and Man since 2005 to 2019, regular teacher Postgraduate TeachingLessons for the Master "Organizing Biological Resources, Course on Biodiversity and Bioindicators of Soil, University of Siena, Prof. Focardi a.a. 1994-1995Professor at the Cortona School of Specialization, Genetic Association of Italy, Genetics of Conservation, a.a. 2003-2004Lessons for the Master “Environmental and Territory Management and Conservation, University of Florence, Sassari and Parma Prof. Apollonio, A.A. 2005-2006Lessons for the Master "Management and Conservation of the Environment and Fauna", University of Florence, Sassari and Parma Prof. Dessì-Fulgheri a.a. 2004-2010Lessons for Postgraduate Course in "Ethology, Animal Welfare and Wildlife Management", University of Florence, Prof. Dessì-Fulgheri a.a. 2007-2010 "
Tutors of many degree Thesis, PhD, fellowships and training period
PERSONAL SKILLS
MOLECULAR LAB-related skills Molecular and cellular biology: Extraction of DNA and RNA from various animal tissues; PCR; RT-PCR; Multiplex PCR; CE-SSCP, RAPDs, mitochondrial and nuclear Sanger sequencing and genotyping; cloning; electrophoresis on agarose gel; spectrophotometric and fluorimetric measure of DNA concentration.
Genetic and genomic data analysis: advanced skills in terminal use of Chromas Softgenetics, Geneious, MEGA, GeneMapper.
Next Generation Sequencing Technologies (NGS): Basic skills for Data analyses of entire transcriptomes by Illumina platforms.
Genetic softwares: Adegenet, Arlequin, Bottleneck, Cervus, DNasp, Fstat, GeneClass, Genepop, Genetix, Modeltest, Structure, Genalex, Poptree, TCS, TCS Buntu, PAUP, Beast, Seaview, Phylips. R-statistics applied to genetic data
Optical and electronic (SEM) MicroscopyImage processing: Adobe Photoshop, Inkscape.Operating systems: Windows, Mac.▪
Driving licence A, B
Mother tongue(s) italian
Linee di ricerca
Uso di tecniche molecolari per la ricostruzionedelle relazioni filogenetiche e per la caratterizzazione della variabilitàgenetica in organismi animali terrestri ed acquatici, sia invertebrati(Crostacei, Insetti) che vertebrati (Uccelli Galliformi, Anseriformi eCharadriformi), in rapporto alla distribuzione geografica, alle capacitàdispersive ed esigenze ecologiche.
Prevenzione dei rischi naturali edantropici: studio delle biocenosi acquatiche sotterranee ai fini dellaprotezione e della salvaguardia delle falde freatiche. Alcuni tra i taxa piùrappresentativi di questo ecosistema (Copepodi e Anfipodi) vengono studiatinella risposta ad agenti inquinanti di origine antropica (fertilizzanti epesticidi), sia da un punto di vista metabolico (respirometria) sia dal puntodi vista molecolare (RT-PCR). A partire dal sequenziamento dell’interotrascrittoma di due specie chiave degli ambienti acquatici di acqua dolce, sonoselezionati geni coinvolti nei meccanismi di detossificazione dell’organismo estudiate le variazioni di espressione in organismi prelevati e mantenuti inassenza di agenti inquinanti (controllo) ed individui sottoposti aconcentrazioni sub letali di varie sostanze e a mix di esse.
Genetica di specie di interesse conservazionistico:vengono studiate specie di Vertebrati di interesse conservazionistico, tra lequali l’occhione (Burhinus oecdinemus, Aves, Charadriiformes), speciepresente in tutto il mediterraneo ed in gran parte dell’Europa e del Nord Africa.Lo scopo è quello di analizzare i pattern genetici in funzione delladistribuzione e del sesso. Vengono studiati loci mitocondriali e loci nuclearidisegnati specificatamente su questa specie. I dati serviranno per comprenderemeglio i movimenti dispersivi e migratori relativi a questa specie e pervalutare le unità di conservazione sulle quali concentrare le attività digestione.
Genetica e selezione sessuale: studiodel Complesso Maggiore di Istocompatibilità (MHC) in relazione ai meccanismi discelta sessuale femminile sulla base della maggiore o minore compatibilitàgenetica tra partners. In specie dimorfiche di Vertebrati dotate di caratterisessuali secondari molto sviluppati il polimorfismo ai loci MHC viene comparatocon la dimensione degli ornamenti sessuali maschili e a caratteristiche qualiil rango e la risposta immunitaria.
Identificazione di specie criptiche estudi di parentela in isopodi (Isopoda, Sphaeromatidae, Sphaeroma terebrans) che abitano i mangrovieti delle regionitropicali e subtropicali.
Legenda
Actual position: Permanent Researcher at CNR
Additional rules
1) Grant Professor at University of Florence “Molecular Zoology” Biologicaland Natural sciences courses
2) Commissary CITES
3) Component Scientific Committee Parco Regionale Migliarino, San Rossore, Massaciuccoli
Educational studies: Degree in Natural Sciences, Department of Animal Biology and GeneticsUniversity of Florence in 1988, with 110/110 cum laude.
Post lauream specialization certificate in Agricultural Acarology, Ohio State University-1989
PHD in AnimalBiology (Zoology) IV° Department of Evolutionary BiologyUniversity of Siena -1992.
Fellowship Tuscany Region-1994-1996
Post-doctoral fellowship Department of Animal Biology and Genetics University of Florence 1996-1998
Research contracts(4 years) Department of Animal Biology and Genetics University of Florence 2000-2004.
Post doctoral international experiences: Syracuse University (NY, USA); Wageningen University, Animal Breeding Group (NL). Academic didactic: Master “Management and conservation of terrestrial fauna” University ofFlorence, Master "Managment of Biological resources", University of Siena; Conservation genetics School of genetics of Cortona (Perugia, Italy); lessons for Zoology in Natural Sciences Courses. Grant professor since 2006 Molecular Zoology at University of Florence
Referee activity (Journal of Biogeography, Animal Genetics, Biological Journal of LinneanSociety, Research for Veterinary Science, Proceedings of the Royal SocietyLondon, series B, Journal of Biogeography, Tropical Zoology, Journal ofBehavioral Ecology and Sociobiology).
Moleculartecnhiques to reconstruct phylogenetic relationships and to characterize thegenetic variability in terrestrial and aquatic animal species, bothInvertebrates (Crustacea, Insecta) and Vertebrates (Aves Galliformes,Anseriformes and Charadriiformes) in relation with geographic distribution,dispersal capabilities and ecological needs.
Geneticsof animal species of conservation concern: Vertebrate species, among them thestone-curlew (Burhinus oecdinemus, Aves, Charadriiformes); arestudied. In particular, the stone-curlew, present in Mediterranean basin, NorthAfrica and the major part of Europe, is analysed in its genetic patterns inrelation with distribution and sex. Mitochondrial and nuclear specific loci arestudied to comprehend the dispersal and migration movements and to individuatethe units of conservation.
Preventionof natural and anthropic risks: study of groundwater biocoenosis for theprotection and saveguard of phreatic layers. Some of the most representativetaxa of this ecosystem (Copepoda and Amphipoda) are studied in the reaction tothe pollutants of human origin (pesticides, fertilizers), both from themetabolic pont of view (respirometry) and genetic (transcriptomes and RT-PCR).Gene involved in the detoxification processes are selected starting from thesequencing of the entire transcriptome of two key species and their expressionpatterns are analysed in individuals in different environmental conditions (notpolluted and pollutted).
Geneticsand sexual selection: Major Histocompatibility Complex (MHC) based female matechoice in relation with genetic compatibility of MHC profiles of the partners.In dimorphic species of Vertebrates equipped by showy Sexual Secondary Traits(SST) the polymorphism at MHC genes is analysed in relation with the dimensionof SSTs and characteristics as the rank or the immune response
Identificationof criptic species and parental studies in Isopods (Isopoda, Sphaeromatidae, Sphaeroma terebrans) living inmangrove ecosystems of tropical and sub tropical regions.