Cognome Pioner | Nome Josè Manuel
Genere M | Data di nascita 26/01/1987 | Luogo di nascita Bolzano-Bozen (BZ) | Nazionalità Italiana
Residente a Firenze (FI)
Email josemanuel.pioner@unifi.it
Pagina web https://www.unifi.it/p-doc2-2021-0-A-2c32382c3b2a-1.html | Twitter @JMPioner
Sede lavorativa Dipartimento di Biologia, Via Madonna del Piano, 6, 50019 Sesto Fiorentino FI | Laboratorio Viale Morgagni 63, 50134, Firenze (Italy)
ISTRUZIONE E FORMAZIONE
17 Febbraio 2016, Dottore di Ricerca in Medicina Molecolare PhD, Corso di Dottorato in Medicina Molecolare, Ciclo XVIII, Università degli studi di Siena
18 Luglio 2012, Dottore Magistrale in Biotecnologie Mediche e Farmaceutiche, Corso di Laurea Magistrale in Biotecnologie Mediche e Farmaceutiche, Università degli studi di Firenze
13 Ottobre 2010, Corso di Laurea Triennale in Biotecnologie, Università degli studi di Firenze
ESPERIENZA LAVORATIVA
3 Marzo 2020 – ad oggi, Ricercatore a tempo determinato tipo A, Dipartimento di Biologia, Università degli studi di Firenze, Firenze, Italy SSD BIO/09
Febbraio 2016 -29 Febbraio 2020, Assegnista di ricerca Postdoc, Dipartimento di Medicina Sperimentale e Clinica, Sezione di Scienze Fisiologiche, Università degli studi di Firenze
Gennaio - Dicembre 2014, Visiting PhD student, Department of Bioengineering, University of Washington, Seattle (WA), USA
Novembre 2012 - 17 Febbraio 2016, Dottorando in Medicina Molecolare, Università degli studi di Siena
Marzo 2012 – Luglio 2012, Studente Erasmus Placement, Institut für Neurophysiologie, Universität zu Köln, Deutschland
SETTORE SCIENTIFICO DISCIPLINARE
BIO/09. Abilitazione Scientifica Nazionale Settore Concorsuale 05/D1 - II Fascia - Dal 02/10/2022 al 02/10/2032
- Docente di Fisiologia generale e comparata, BIO/09, Corso di Laurea in Scienze Naturali (Università degli Studi di Firenze);
- Docente di Fisiologia, BIO/09, Corso di Laurea Triennale in Biotecnologie (Università degli Studi di Firenze).
AREA DI COMPETENZA SCIENTIFICA
Fisiologia del muscolo cardiaco, Biochimica, Genetica, Biologia Molecolare, Farmacologia, Biomateriali (fonte Scopus, ORCID: http://orcid.org/0000-0003-4878-4820)
PRODUZIONE SCIENTIFICA
• Scopus Author ID: 57035436600
• ORCID: https://orcid.org/0000-0003-0179-3033
• Pubmed https://www.ncbi.nlm.nih.gov/pubmed/?term=Pioner+JM
- European Horizon 2020 (grant 777204): In Silico trials for drug tracing the effects of sarcomeric protein mutations leading to familial cardiomyopathy, SILICO-FCM. Collaborazioni con University of Washington (USA), University of Kent (UK), Illinois Institute of Technology (USA).
-Telethon-UILDM funded project (GUP19012). “Towards Precision Medicine with Human Induced Pluripotent Stem Cells for Dystrophin Associated Cardiomyopathy”. Collaborazione con Centro Cardiologico Monzino (Milano, IT), Policlinico Gemelli (Roma, IT), LENS (Sesto Fiorentino, IT)
-Telethon funded project (GGP16191): “A novel in vitro Duchenne Muscular Dystrophy cardiomyopathy model: human iPSC-derived cardiomyocytes for mechanistic studies”. Collaborazione con LENS (Sesto Fiorentino, IT)
INTERESSI DI RICERCA
Fisiologia del muscolo cardiaco: accoppiamento eccitazione contrazione cardiaco, contrazione muscolare, proprietà contrattili di singole miofibrille, elettrofisiologia, transienti di calcio;
Modelli sperimentali in vitro: cellule staminali pluripotenti indotte (iPSC), ingegneria tissutale;
Meccanismi cellulari alla base di cardiomiopatie genetiche: cardiomiopatia ipertrofica, dilatativa, distrofia muscolare.
Legenda
Name Josè Manuel | Surname Pioner
Sex M | Date of Birth January 26, 1987 | Place of Birth Bolzano-Bozen (BZ) | Nationality Italian
Resident in Firenze (FI)
Web page https://www.unifi.it/p-doc2-2021-0-A-2c32382c3b2a-1.html | Twitter @JMPioner
Working address Dipartimento di Biologia, Via Madonna del Piano, 6, 50019 Sesto Fiorentino FI | Laboratory Viale Morgagni 63, 50134, Firenze (Italy)
EDUCATION AND TRAINING
-February 17, 2016 - PhD in Molecular Medicine Cycle XVIII, University of Siena
-July 18, 2012 - Master degree in Pharmaceutical and Medical Biotechnology, University of Florence
-13, 2010 – Bachelor's degree in Biotechnology in Biotechnology, University of Florence
WORK EXPERIENCE
March 3, 2020 - to date FIXED-TERM RESEARCHER TYPE A (named as “Ricercatore a tempo determinate di tipo A, RtdA”) Department of Biology, University of Florence
September 1, 2018 – March 3, 2020 POSTDOCTORAL FELLOW Department of Experimental and Clinical Medicine, Division of Physiology, University of Florence ▪ Horizon 2020 (grant 777204) European project: In Silico trials for drug tracing the effects of sarcomeric protein mutations leading to familial cardiomyopathy, SILICO-FCM. SSD BIO/09. Coordinator: Corrado Poggesi
April 2017-September 2018 POSTDOCTORAL FELLOW Department of Experimental and Clinical Medicine, Division of Physiology, University of Florence ▪ Telethon project (GGP16191): “A novel in vitro duchenne muscular dystrophy cardiomyopathy model: human ipsc-derived cardiomyocytes for mechanistic studies” Prot. N.22784(1706) Unifi. SSD BIO/09 Duration: 16 months. Coordinator: Cecilia Ferrantini
February 2016 – April 2017 POSTDOCTORAL FELLOW Department of Experimental and Clinical Medicine, Division of Physiology, University of Florence ▪ Project: “Messa a punto e caratterizzazione di un modello cellulare in coltura da precursori cellulari muscolari umani per studi farmacologici, funzionali e molecolari” Prot. n. 115440 of 10/09/2015 D.D. n. 4822 Unifi. Duration: 14 months. SSD BIO/09. Coordinator: Corrado Poggesi
January - December 2014 VISITING PhD STUDENT Department of Bioengineering, University of Washington, Seattle (WA), USA Supervisor: Prof. Micheal Regnier Collaborations: Charles E. Murry, Martin K. Childers, David L. Mack, Deok-Ho Kim
November 2012- February 17, 2016 PhD CANDIDATE IN MOLECULAR MEDICINE University of Siena Supervisor: Corrado Poggesi (University of Florence)
SCIENTIFIC DISCIPLINARY SECTOR
BIO/09. National Scientific Habilitation, Academic Field for Italian University National Recruitment 05/D1 - II Level- valid from 02/10/2022 to 02/10/2032
- Lecturer of General and Comparative Physiology, School of Mathematics, Physics and Natural Sciences, Natural Science course, University of Florence
- Lecturer of Physiology, School of Human Health Sciences, Biotechnology course, University of Florence
RESEARCH AREA
Cardiac muscle physiology, Biochemistry, Genetic and Molecular Biology, Biomaterial science, Pharmacology (source Scopus, ORCID: http://orcid.org/0000-0003-4878-4820)
SCIENTIFIC PRODUCTION
- Scopus Author ID: 57035436600
- ORCID: http://orcid.org/0000-0003-4878-4820
-Pubmed https://www.ncbi.nlm.nih.gov/pubmed/?term=Pioner+JM
-European Horizon 2020 (grant 777204): In Silico trials for drug tracing the effects of sarcomeric protein mutations leading to familial cardiomyopathy, SILICO-FCM. Coordinator: Corrado Poggesi. Collaboration with: Michael Regnier (University of Washington), Mike Geeves (University of Kent), Srba Mijailovich (Illinois Institute of Technology). In this context, he spent two months (July-August 2019) in Michael Regnier lab (University of Washington).
-Telethon-UILDM funded project (GUP19012). “Towards Precision Medicine with Human Induced Pluripotent Stem Cells for Dystrophin Associated Cardiomyopathy”. Coordinator: Giulio Pompilio Partners: Cecilia Ferrantini (UNIFI), Leonardo Sacconi, Filippo Crea. Generation of an Italian biobank of blood and hiPSCs from patients with dystrophin associated cardiomyopathy
-Telethon funded project (GGP16191): “A novel in vitro Duchenne Muscular Dystrophy cardiomyopathy model: human iPSC-derived cardiomyocytes for mechanistic studies”. Coordinator: Cecilia Ferrantini. Parnter: Leonardo Sacconi (LENS). Electrophysiological and mechanical assessment of DMD patient and CRISPR-Cas9- hiPSC-cardiomyocytes. In this context, he spent two months in the Barbuti lab (University of Milan) collaborating with Patrizia Benzoni.
- Coordinator of the 20223L2C9N project “The Role of Cardiac Extracellular matrix - Cell interaction in the onset of dystrophic cardiomyopathies (MALADAPT)” 2023-25 (Call: Bando PRIN 2022 Ministero dell'Università e della Ricerca)(Unit: Dr. Patrizia Benzoni, Università degli studi di Milano)
- Unit PI of the GR-2021-12375403 project “Precision medicine in dystrophin cardiomyopathies - characterization of induced pluripotent stem cells (iPSC) derived from Duchenne patients with advanced heart failure using artificial intelligence to differentiate disease pathways and identify predictors for treatment response”. 2023-2026 (Call: Bando di Ricerca Finalizzata ai Giovani Ricercatori - Ministero della Salute della Repubblica Italiana) (Units: Dr. Domenico D’Amario (coordinator) and Daniela Leone from Gemelli Hospital, Rome (IT) and Dr. Davide Rovina from Centro Cardiologico Monzino, Milano (IT)
- Unit PI of the G23_63 project “Modeling DMD-associated Cardiomyopathy Using Patient-specific Derived Cardiomyocytes” between Università degli Studi di Firenze e Université de Montpellier. 2023-2024 (Call: Galileo 2023 from the Université franco-italienne) (Partner: Dr. Meli Albano from CRCN Inserm, Montpellier (FR)).
- Unit PI of the project “At the molecular roots of arrhythmias in genetic cardiomyopathies: preventing electrophysiological remodelling with novel therapeutic strategies.” University grant, Call: “Bando di ateneo progetti competitivi per Ricercatori a tempo determinato” (Università degli studi di Firenze). Coordinator: Dott. Raffaele Coppini, Dipartimento di Neurofarba, UNIFI). 2022-2023 ongoing
Physiology of cardiac muscle: cardiac excitation-contraction coupling; mechanics of cardiac muscle from human and rodent models; mechanics and energetics of skinned cardiac muscle and single myofibrils (sarcomere regulation); optical recording using fluorescent indicators (calcium- and voltage- sensitive dyes)
Research models: human cardiac tissue (myectomies) and cardiomyocytes, murine cardiac muscle and cardiomyocytes, pluripotent stem cells, pluripotent stem cell-derived cardiomyocytes, smart biomaterials
Genetic Cardiomyopathies: Hypertrophic and Dilated Cardiomyopahty, Muscular Dystrophies
