B012821 - INSTRUMENTAL METHODS IN ANALYTICAL CHEMISTRY

Electroanalysis: potentiometric and voltammetric tecniques. Spectrophotometric analysis. Optical and electrochemical sensor. Cromatography. New analytical methods. Development of biosensors and their applications. Experimental error. Statistics for analytical measurements. Analytical Quality Criteria and performance tests. Calibration. Signaling processing. Sampling and sample preparation for analysis. Selected laboratory experiments on the Course topics.

Chimica Analitica Strumentale, Rubinson, Rubinson, Zanichelli. Chimica Analitica Strumentale, Skoog, Leary, EdiSES

Knowledge acquired: The course supplies to the student sound knowledge of the theory and treatment of the data obtained using the modern analytical techniques. Selected laboratory practices on the course topics will held.
Competence acquired This course will give students an overview of the modern analytical instrumental methods. The student will know the equipment available to analysts, the errors inherent in all results and statistical ways of handling those results and their errors.
Skills acquired (at the end of the course):This course will provide a broad understanding and “hands-on” knowledge of the advanced methods in analytical chemistry.

Courses recommended: Sensors and biosensors

Total hours of the course (including the time spent in attending lectures, seminars, private study, examinations, etc...): 150
Hours reserved to private study and other indivual formative activities: 80
Contact hours for: Lectures (hours): 36
Contact hours for: Laboratory (hours): 30
Contact hours for: Laboratory-field/practice (hours): 14

Office hours:
Monday morning

Written and Oral examinations

Application of statistical methods in analytical chemistry. Validation and quality assurance processes. Performance characteristics; the limitations imposed by instrument design; estimation of uncertainties in instrumental measurement; and specification of equipment for purchase. Electroanalytical methods. Potentiometry and Ion Selective Electrodes. Amperometry and voltammetry. Elettroanalysis. Fundamentals of separation tecniques. Gas Chromatography. The topic will give an appreciation of the essentials of gas chromatography as an analytical tool and an understanding of the main causes of errors in analyses. High Performance Liquid Chromatography. This topic covers the foundations of liquid chromatography, the equipment needed for HPLC, column packings, and the different applications of the technique. Ionic chromatography:theory and instrumentation. Size Exclusion Chromatography. Elettrophoresis. Capillary electrophoresis. Biological molecules detections. Immunochemistry. Theory of immunology. ELISA assay. Detection of mutagens. Sensor and biosensor. Applications in clinical, food and environmental fields. Automation, miniaturization. Microfabrication. Miniaturized analytical system. New nanomaterials for analytical applications. Automatic methods for analysis. Microfluidic system, Lab on chip. Selected laboratory experiments on the Course topics.